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Our Discovery Biology department offers services supporting structure-based drug discovery from determination of novel targets to final structures. Our platform is one of the earliest established structural biology platforms in China and has been certified by the Shanghai Government.
Biochemical Assay
Kinase Assay: VEGFR2 Inhibition Using HTRF
Protease Assay: Renin inhibition using FRET
Validated Biochemical Assay List
Validated Enzymatic Targets | |||
Abl | COX-1/COX-2 | HER2 | PI3K alpha |
AKT1 | C-Raf | IKK beta | PI3K beta |
AKT2 | DPPIV | JAK1 | PI3K delta |
AKT3 | EGFR | JAK2 | PI3K gamma |
ALK | EGFR (L858R,T790M) | JAK3 | PKA |
Aurora A | Factor Xa | LCK | PKC delta |
Aurora B | FGFR1 | Lipoxygenase | PKC gamma |
B-Raf | Flt1/VEGFR1 | Lyn | Plk1 |
BTK | Flt3 | MAPK14 | 20S proteasome |
Cathepsin K/L/B/S | Flt4 | MARK1 | Renin |
CDK/P25 | α- Glucosidase | MEK1 | SAHase |
CHK1 | β- Glucosidase | MEK2 | SGK1 |
CHK2 | GSK3 beta | mTOR | SRC |
c-Kit | H+/K+ ATPase | PDE5 | Syk |
c-Met | HDAC1 &HDACs | PDGFR beta | VEGFR2 |
Note: The enzyme in blue is Kinase |
The
enzyme in blue is Kinase
Cellular-based Assay
Cytotoxicity Assay Using celltiter glo
Cell-based Cathepsin Assay for irreversible inhibitors
Cell Cycle Analysis Using Flow Cytometry
Western Blot Analysis
Expression level of Phospho-Akt and Akt in mouse tumor
Migration Assay in HT1080 cells and MDA-MB-231 cells
Cytotoxicity Assay Using celltiter glo
Cell-based Cathepsin Assay for irreversible inhibitors
Pre-migration |
Post-migration |
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Background |
Positive control |
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Radioactive Assay
Receptor binding assay-3H
3H-5HT uptake assay-3H
Radioactive iodine uptake assay-125I
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