Medicilon established a well-developed structural biology research platform. Medicilon is proud as the first CRO to provide gene to crystal structure service in China. We have well-established integrated service platform, which has been certified as an important platform for drug discovery by Shanghai Government.
Medicilon has more than ten years of experiences in providing gene to crystal structure service, including construction design, protein expression and purification, high-throughput screen of crystallization conditions, compound or fragments soaking and structure determination.
|1||Crystallization condition screen by Mosquito||4-8 weeks||Commercial screen kits, Sparse matrix screen, 18 oC and 4 oC|
|2||X-ray diffraction data collection at Synchrone||4-8 weeks||Collected at SSRF weekly|
A. Co-crystallization of Protein-compound
B. Fragment Screen by soaking or co-crystallization
|4-8 weeks||Binding affinity date of compounds with protein is crucial|
|4||Determination of structure||TBD||Phase solved by MR, SeMet or MIR|
Remarks: The service is highly flexible to meet your request. One or more above steps could be separately provided.
|Build the homology model of PI3K delta||Compared 3D structures of PI3K isoforms, identify critical residues and pocket features for selectivity and affinity||Compounds were designed based on protein structures|