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E. coli expression system is the earliest developed and most widely used gene expression system. In recent decades, E.coli expression system has been popularly used and developed for producing a wide variety of different types of proteins. Compared with other expression systems, E. coli is a well-established host with the advantages of high gene expression level, short cultivation time, strong anti-polluted ability and relatively low cost.
Researchers of Medicilon established a well-developed E. Coli Expression and Purification Services Platform, which offers the expression and purification services of a variety of recombinant proteins.
E. coli expression system is the earliest developed and most widely used gene expression system. In recent decades, E.coli expression system has been popularly used and developed for producing a wide variety of different types of proteins. Compared with other expression systems, E. coli is a well-established host with the advantages of short culturing time, easy genetic manipulation and low cost media.
Medicilon researchers established a well-developed E. coli expression and purification services platform, which offers the expression and purification services of a variety of recombinant proteins, with a fast turn-around time and competitive pricing.
Steps | Items | Timeline | Comments |
1 | Generation of Recombinant Plasmid | 2 weeks | Multiple expression vector, different affinity tag: His, Trx , GST, SUMO… |
2 | Expression of Recombinant Protein | 1 week | Multiple expression host |
3 | Soluble Expression Optimization | 1-2 weeks | Test different induction concentration, time point, temperatures or reconstruct plasmids |
4 | Small Scale Expression and Purification | 1-2 weeks | Purification of soluble expression or from inclusion body |
5 | Scale Up Expression and Purification | TBD | Culture in flask or fermenter |
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